Generator
SAG

Part:BBa_J45300

Designed by: MIT IGEM 2006   Group: iGEM06_MIT   (2006-08-21)

Salicylic acid generator

The salicylic acid generator (BBa_J45300) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45319). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.

Usage and Biology

BBa_J45300 is almost identical to BBa_J45320 except that it uses a different ribosome binding site (BBa_B0030 instead of BBa_B0032).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 920
    Illegal NgoMIV site found at 931
    Illegal NgoMIV site found at 1212
    Illegal NgoMIV site found at 1680
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None